Localisation of intracellular calcium stores in the striated muscles of the jellyfish Polyorchis penicillatus: possible involvement in excitation-contraction coupling

When jellyfish striated muscles were stimulated directly, the amplitude of contractile tension increased as the stimulation frequency increased. Appli cation of 10 mmol l(-1) caffeine reduced the amplitude of contractile tensi on and abolished this facilitatory relationship, indicating that calcium st ores participate in excitation-contraction coupling. Calcium stores were id entified ultrastructurally using enzymatic histochemistry to localize CaATP ases, and potassium dichromate to precipitate calcium. Electron energy-loss spectroscopy was used to verify the presence of calcium in precipitates. B oth CaATPase and calcium were localised in membrane-bound vesicles beneath the sarcolemma. We concluded that sub-sarcolemmal vesicles could act as cal cium stores and participate in excitation-contraction coupling.