A sensitive and reproducible method for the detection of oligoclonal IgM bands

Authors
Villar, LM Gonzalez-Porque, P Masjuan, J Alvarez-Cermeno, JC Bootello, A Keir, G
Citation
Lm. Villar et al., A sensitive and reproducible method for the detection of oligoclonal IgM bands, J IMMUNOL M, 258(1-2), 2001, pp. 151-155
Citations number
7
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGICAL METHODS
ISSN journal
00221759 → ACNP
Volume
258
Issue
1-2
Year of publication
2001
Pages
151 - 155
Database
ISI
SICI code
0022-1759(200112)258:1-2<151:ASARMF>2.0.ZU;2-W
Abstract
We have developed a sensitive and specific isoclectrofocusing (IEF) method for the detection of oligoclonal (OGC) IgM in the cerebrospinal fluid (CSF) and sera. In this procedure, ampholytes, in the range pH 5-8, were used to improve the resolution, and serum was diluted in saline to avoid IgM preci pitation. Samples were treated with 50 MM dithiothreitol (DTT) at pH 9.5 to reduce lgM and improve the migration of the protein. Using an anti-IgM lab elled with alkaline phosphatase, the limit of detection was found to be 0.1 ng in 5 mul of sample. (C) 2001 Elsevier Science B.V. All rights reserved.