Cutting edge: Histone acetylation and recombination at the TCR gamma locusfollows IL-7 induction

IL-7 signaling is required for V(D)J recombination at the TCR gamma locus. We have recently reported that IL-7 controls chromatin accessibility for RA G-mediated cleavage. Inhibition of histone deacetylase substituted for the IL-7 signal, indicating a role for histone acetylation in altering chromati n accessibility. We found a greatly reduced histone 3 and histone 4 acetyla tion level in IL-7R alpha (-/-) thymocytes in comparison with RAG(-/-) thym ocytes or fetal thymocytes. Sterile transcripts, indicating an open chromat in configuration, were suppressed in IL-7R alpha (-/-) and IL-7(-/-)RAG(-/- ) thymocytes. Moreover, exogenously added IL-7 induced sterile transcripts from the TCR gamma constant region in cultured thymocytes from IL-7(-/-)RAG (-/-) mice. This induction correlated with increased histone acetylation at the J-promoter and C-enhancer regulatory elements at the TCR gamma locus. These results suggest that IL-7 regulates chromatin accessibility for V(D)J recombination by specifically altering histone acetylation within the TCR gamma locus.