R. Oberbauer et al., Regulation of renal tubular cell apoptosis and proliferation after ischemic injury to a solitary kidney, J LA CL MED, 138(5), 2001, pp. 343-351
Citations number
31
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research General Topics
The time course and regulation of apoptosis and cellular regeneration after
30 minutes of acute ischemic injury to a single kidney was elucidated in r
ats at five time points over 20 weeks. The fraction of apoptotic cells was
most prominent at 1 day after the insult in the distal tubule (8% +/- 4% vs
. 0% +/- 0%, acute renal failure (ARF) vs sham, respectively) and was still
elevated at 7 days (2% +/- 2% vs 0% 0%). At that time, the whole kidney mR
NA expression of the apoptosis inhibitory genes bcl-xL and bcl-2, as well a
s that of the apoptosis promotor box, was significantly reduced. Immunohist
ochemistry of kidney specimen showed suppression of bcl-2 in the distal tub
ule but up-regulation in the proximal tubule, whereas bax protein was more
strongly expressed in the distal tubule. Cellular proliferation started at
day 1 and continued over the following 20 weeks, leading to severe tubular
dilation and kidney failure. These data indicate that differential regulati
on of bcl-2 family members contributes to the early apoptotic clearance of
lethally injured tubular epithelial cells after ischemic injury to a solita
ry kidney.