Determination of furfural compounds in enteral formula

HPLC methods are described for the determination of furanic compounds (hydr oxymethylftufural and furfural) in enteral formulas prepared with dextrinom altose and milk proteins, and in model systems enclosing these ingredients. These compounds were extracted in aqueous solution, purified with organic solvents, and separated in a reversed-phase C-18 column with water-acetonit rile (95:5 v/v). Average recovery rates of HMF and furfural were 99.2% and 71.1%, respectively. The variation coefficients for HMF and furfural were 2 .41% and 1.23%, respectively. The detection limit was 0.01 mg/L for both co mpounds. HMF and furfural levels in enteral formulas ranged from 0.05 to 19 .1 mg/L and from 0.14 to 0.72 mg/L, respectively.