Biomimetic coatings functionalized with adhesion peptides for dental implants

A complete biological integration into the surrounding tissues (bone, gingi va) is a critical step for clinical success of a dental implant. In this wo rk biomimetic coatings consisting either of collagen type I (for the gingiv a region) and hydroxyapatite (HAP) or mineralized collagen (for the bone in terface) have been developed as suitable surfaces regarding the interfaces. Additionally, using these biomimetic coatings as a matrix, adhesion peptid es were bound to further increase the specificity of titanium implant surfa ces. To enhance cell attachment in the gingiva region, a linear adhesion pe ptide developed from a laminin sequence (TWYKIAFQRNRK) was bound to collage n, whereas for the bone interface, a cyclic RGD peptide was bound to HAP an d mineralized collagen using adequate anchor systems. The biological potent ial of these coatings deduced from cell attachment experiments with HaCaT h uman keratinocytes and MC3T3-E1 mouse osteoblasts showed the best results f or collagen and laminin sequence coating for the gingiva region and mineral ized collagen and RGD peptide coatings for regions with bone contact. Our c oncept opens promising approaches to improve the biological integration of dental implants. (C) 2001 Kluwer Academic Publishers.