J. Svitel et al., Functionalized surfaces for optical biosensors: Applications to in vitro pesticide residual analysis, J MAT S-M M, 12(10-12), 2001, pp. 1075-1078
Citations number
20
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Functionalized biosensing surfaces were developed for chemiluminescent immu
noassay of pesticides. Two approaches to construct functionalized surfaces
were tested: (i) pesticide is immobilized to the surface and interacts with
a labeled antibody; (ii) antibody is immobilized and interacts with a labe
led pesticide. As labels alkaline phosphatase and peroxidase were used with
their corresponding substrates CSPD and luminol, respectively. Light produ
ced by chemiluminescent substrate was detected by a thermoelectrically cool
ed CCD camera or a photomultiplier. The best detection limit 0.00001 ng/ml
was obtained using antibodies immobilized to dextran-enhanced surface. Comp
letely renewable surface was obtained using reversible lectin-monosaccharid
e interaction, one surface was used for 200 analyses without any loss of bi
nding capacity. Most favorable stability and cost per analysis was achieved
with molecularly imprinted polymer (MIP) instead of antibody. The function
alized biosensing surfaces were prepared to detect 2,4-dichlorophenoxyaceti
c (2,4-D) acid as a model pesticide. The developed concepts are, however, g
enerally applicable to other pesticides and to other optical formats, e.g.
optical fiber. (C) 2001 Kluwer Academic Publishers.