Glucose biosensors based on dendrimer monolayers

The peculiarities of glucose biosensors based on different generation of de ndrimers (G0, G1 and G4) have been studied by amperometry and QCM technique s. It is shown that stable glucose biosensor can be obtained with low gener ation of dendrimers. The sensor sensitivity, however considerable, increase d with increasing number of generation of dendrimers. This can be due to th e increased volume of the dendrimer interior as well as with increased numb er of binding sites for glucose oxidase (GOX). QCM experiments showed that immobilization of GOX resulted in formation of enzyme multilayers on a dend rimer surface. The enzyme turnover for this system (0.1-0.01 s(-1)) was low er then that for immobilization of GOX onto a supported lipid films by mean s of avidin-biotin technology (1.1 s(-1)). However, dendrimer based biosens ors are more stable in comparison with sBLM based sensors and could be stor ed in a refrigerator in dry conditions over 15 days without substantial los s of sensitivity. (C) 2001 Kluwer Academic Publishers.