GeneChip((R)) analysis after acute spinal cord injury in rat

Spinal cord injury (SCI) leads to induction and/or suppression of several g enes, the interplay of which governs the neuronal death and subsequent loss of motor function. Using GeneChip (R), the present study analyzed changes in the mRNA abundance at 3 and 24 h after SCI in adult rats. SCI was induce d at T9 level by the New York University impactor by dropping a 10-g weight from a height of 25 mm. Several transcription factors, immediate early gen es, heat-shock proteins, pro-inflammatory genes were up-regulated by 3 h, a nd persisted at 24 h, after SCI. On the other hand, some neurotransmitter r eceptors and transporters, ion channels, kinases and structural proteins we re down-regulated by 3 h, and persisted at 24 h, after SCI. Several genes t hat play a role in growth/differentiation, survival and neuroprotection wer e up-regulated at 24 h after SCI. Using real-time quantitative PCR, the cha nges observed by GeneChip (R) were confirmed for seven up-regulated (interl eukin-6, heat-shock protein-70, heme oxygenase-1, suppressor of cytokine si gnaling 2, suppressor of cytokine signaling 3, interferon regulatory factor -1, neuropeptide Y), two down-regulated (vesicular GAGA transporter and cho lecystokinin precursor) and two unchanged (Cu/Zn-superoxide dismutase and p hosphatidyl inositol-3-kinase) genes. The present study shows that inflamma tion, neurotransmitter dysfunction, increased transcription, ionic imbalanc e and cytoskeletal damage starts as early as 3 h after SCI. In addition to these effects, 24 h after SCI the repair and regeneration process begins in an attempt to stabilize the injured spinal cord.