LC method for the analysis of paracetamol, caffeine and codeine phosphate in pharmaceutical preparations

An accurate. simple, reproducible and sensitive method for the determinatio n of paracetamol, caffeine and codeine phosphate has been developed and val idated. Paracetamol, caffeine and codeine phosphate were separated using a mu Bondapack C-8 column by isocratic elution with flow rate 1.0 ml/min. The mobile phase composition was 420/20/30/30 (v/v/v/v) 0.01 M KH2PO4, methano l, acetonitrile, isopropyl alcohol and spectrophotometric detection was car ried out at 215 nm. The linear range of detection for paracetamol, caffeine and codeine phosphate were between 0.400 and 1500 mug/ml: 0.075 and 90 mug /ml: 0.300 and 30 mug/ml, respectively. The method has been shown to be lin ear, reproducible, specific, sensitive and rugged. (C) 2001 Elsevier Scienc e B.V. All rights reserved.