Characterization of micellar solubilization of antibiotics using micellar electrokinetic chromatography

Micellar electrokinetic chromatography (nMEKC) method was applied to the de termination of the partition behaviour between water and micelle for a grou p of antibiotics using sodium dodecyl sulphate (SDS) as an anionic model su rfactant. In the method, the running buffer contains both the micelles and the drug, and the injected solution contains the same concentration of the micelles as the running buffer but no drug. The mobility of the drug can be measured from a negative peak recorded in the chromatogram. The required p arameters for the determination of the capacity factor were measured by the MEKC technique. Thermodynamic properties such as enthalpy and entropy chan ges of micellar solubilization were calculated from the temperature depende nce of the partition coefficients. The critical micellar concentrations (CM C) of the SDS salt were determined in phosphate solutions at pH 7 at differ ent temperatures using the MEKC. The method described in this article based on MEKC is efficient and very fast in order to determine parameters for ch aracterizing micellar solubilization of drugs. (C) 2001 Elsevier Science B. V. All rights reserved.