Preparation and evaluation of I-125-aflatoxin B-1

Aflatoxin B-1 (AfB(1)), present in fungus infested crops is highly carcinog enic and is measured by immunoassays. I-125 labeled aflatoxin B-1 is a key reagent for development of radioimmunoassay (RIA) which exhibits less inter ference and better sensitivity than other immunoassays. Since AfB(1) lacks suitable functional groups for radiolabeling, an oxime derivative of AfB(1) was synthesised and evaluated by UV-spectrophotometry and H-1 NMR spectros copy. I-125-histamine was conjugated to AfB(1) oxime by mixed anhydride met hod and purified by solvent extraction followed by TLC. The tracer obtained was immunoreactive, stable as ethanolic solution and could be used in RIA.