Feasibility of quantitative determination of doxorubicin with surface-enhanced Raman spectroscopy

Surface-enhanced Raman spectroscopy (SERS) was performed using excitation a t 488 nm in a blood plasma-doxorubicin-silver colloid system. With a blood plasma content of 1%, a partial least-squares calibration of the doxorubici n was made in the 10-750 nm range. Predictions for a test set generated a r oot mean square error of prediction of 70 nm. The use of SERS and chemometr ics in complex systems made it possible to use the highly informative Raman signals even at low concentrations without the need for sample pretreatmen t such as extraction. Copyright (C) 2001 John Wiley & Sons, Ltd.