S. Kim et al., Evaluation of Kang-Fung-Sol medium for selectivity of lactic acid bacteriafrom laboratory medium and ground beef, J RAPID M A, 9(3), 2001, pp. 161-169
Citations number
8
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY
deMan Rogosa Sharpe (MRS) medium has usually been used for the recovery of
lactic acid bacteria (LAB) from various food products. However, it also all
ows non-LAB to grow due to its nutritive ingredients. Three kinds of agar m
edia, MRS, Kang-Fung (KF), and Kang-Fung-Sol (KFS), were evaluated for sele
ctive growth of LAB from mixed cultures. All bacteria tested grew well on M
RS medium. KF medium was effective for the inhibition of gram-negative bact
eria, but not for some gram-positive bacteria. KFS medium completely inhibi
ted the growth of all non-LAB tested. Furthermore, the growth of LAB tested
on KFS medium was not significantly different from that on MRS or KF mediu
m. Microbial counts from inoculated ground beef of Pediococcus pentosaceus,
Escherichia coli O157:H7, Pseudomonas aeruginosa, and Salmonella arizonae
did not show a significant difference between MRS and KF medium. However, n
o non-LAB were recovered from ground beef using KFS medium. Ae recovery of
Pediococcus pentosaceus was not significantly different on all three media
tested. This research shows that KFS medium can be used to isolate and enum
erate LAB in liquid medium or ground beef in the presence of other microorg
anisms.