Physical and transcriptional map of the mouse Chromosome 10 proximal region syntenic to human 6q16-q21

Toward the isolation of the grey-lethal (gl) gene, we have genetically loca lized this locus on mouse Chromosome (Chr) 10 between the Fyn gene and the D10Mit148 microsatellite marker. Here, we have screened five yeast artifici al chromosome (YAC) libraries and isolated more than 100 YAC clones mapping to this region. Forty-two clones were characterized and assembled in an si milar to8.5 me-abases (Mb) contig showing high linkage conservation with th e human 6q16-q21 interval. During this study, 24 specific novel sequence-ta gged sites (STSs) were derived from YAC insert ends, and 15 mouse genes wer e precisely mapped to the contig. The physical and transcriptional map pres ented here will provide novel resources to isolate the gl locus associated with osteopetrosis, and will also provide candidate loci for other defects mapped on human Chr 6q.