The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshift

We have determined the effect of environmental factors (mild thermal upshif t and starvation) on the Candida albicans cell wall-associated glyceraldehy de-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degreesC) and/or starvation (at 28 or 37 degreesC in water) of exp onentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not corr elate with an increase in the amount of cwGAPDH protein present, as determi ned by flow cytometry, immunoelectron microscopy and Western-blotting. Thes e results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. albicans cells.