M. Taneyama et al., Involvement of gibberellins in expression of a cysteine proteinase (SH-EP)in cotyledons of Vigna mungo seedlings, PLANT CEL P, 42(11), 2001, pp. 1290-1293
The expression of a papain-type proteinase, designated SH-EP, in cotyledons
of Vigna mungo seedlings has been shown to require some factors in the emb
ryonic axes. Gibberellin Al (GA,) and GA(20) were identified by GC-MS in em
bryonic axes of V mungo seedlings. The level of accumulation of SH-EP in co
tyledons of V mungo seedlings was greatly reduced by treatment of the seeds
with uniconazole-P, an inhibitor for GA biosynthesis. The reduced level of
accumulation of SH-EP in cotyledons by uniconazole-P was recovered by exog
enous application of GA(1) and GA(20) to the seedlings.