Ma. Farmer et al., A major quantitative trait locus on chromosome 3 controls colitis severityin IL-10-deficient mice, P NAS US, 98(24), 2001, pp. 13820-13825
Citations number
37
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Colitic lesions are much more severe in C3H/HeJBir (C3H) than C57BL/6J (B6)
mice after 10 backcrosses of a disrupted interleukin-10 (1/10) gene. This
study identified cytokine deficiency-induced colitis susceptibility (Cdcs)
modifiers by using quantitative trait locus (QTL) analysis. A segregating F
-2 population (n = 408) of IL-10-deficient mice was genotyped and necropsie
d at 6 weeks of age. A major C3H-derived colitogenic QTL (Cdcs1) on chromos
ome (Chr.) 3 contributed to lesions in both cecum [logarithm of odds ratio
(LOD) = 14.6)] and colon (LOD = 26.5) as well as colitis-related phenotypes
such as spleen/body weight ratio, mesenteric lymph node/body weight ratio,
and secretory IgA levels. Evidence for other C3H QTL on Chr. 1 (Cdcs2) and
Chr. 2 (Cdcs3) was obtained. Cdcs1 interacted epistatically or contributed
additively with loci on other chromosomes. The resistant B6 background als
o contributed colitogenic QTL: Cdcs4 (Chr. 8), Cdcs5 (Chr. 17, MHC) and Cdc
s6 (Chr. 18). Epistatic interactions between B6 QTL on Chr. 8 and 18 contri
buting to cecum hyperplasia were particularly striking. In conclusion, a co
litogenic susceptibility QTL on Chr. 3 has been shown to exacerbate colitis
in combination with modifiers contributed from both parental genomes. The
complex nature of interactions among loci in this mouse model system, coupl
ed with separate deleterious contributions from both parental strains, illu
strates why detection of human inflammatory bowel disease linkages has prov
en to be so difficult. A human ortholog of the Chr. 3 QTL, if one exists, w
ould map to Chr. 4q or 1p.