Autonomous replication of a Bombyx mori transgene fragment in the yeast Saccharomyces cerevisiae

Authors
Legchilina, SP Efimenko, IG Aniskina, YV Arman, IP Tarantul, VZ Nikolaev, AI
Citation
Sp. Legchilina et al., Autonomous replication of a Bombyx mori transgene fragment in the yeast Saccharomyces cerevisiae, RUSS J GEN, 37(11), 2001, pp. 1257-1265
Citations number
60
Categorie Soggetti
Molecular Biology & Genetics
Journal title
RUSSIAN JOURNAL OF GENETICS
ISSN journal
10227954 → ACNP
Volume
37
Issue
11
Year of publication
2001
Pages
1257 - 1265
Database
ISI
SICI code
1022-7954(200111)37:11<1257:AROABM>2.0.ZU;2-N
Abstract
A previously cloned autonomous transgene (pr8a) of silkworm Bombyx mori inh erited without changes in the structure was used to clarify the activity of its ARS in yeast cells. ARS of pr8a was also shown to maintain autonomous replication of hybrid plasmids in yeast cells. The same was true for its ce ntral 2.4-kb fragment devoid of flanking sequences.