Reverse in situ hybridization of DNA probes of abnormal chromosomes in diagnostics of chromosomal pathologies

The results of analysis of congenital chromosomal pathologies and chromosom al rearrangements upon the occurrence of haematological diseases, which was involved constructing DNA libraries of abnormal chromosomes and subsequent reverse CISS hybridization have been considered. High effectiveness of thi s approach for analysis of chromosomal translocations, deletions of chromos omal regions, minor additional chromosomes, and large marker chromosomes wi th complex organization was shown. The possibility of implementation of thi s approach and its large-scale application in medical and genetic studies o f congenital developmental pathologies and chromosomal diagnostics of haema tological diseases has been discussed.