G. Servais et al., Diagnostic specificities and sensitivities of anti dsDNA, anti membrane DNA and anti nucleosomes autoantibodies, SC J CL INV, 61, 2001, pp. 61-67
Citations number
31
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research General Topics
Journal title
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
The aim of this study is to evaluate, from 369 routine sera of SLE and cont
rol patients, the worth of anti double stranded nuclear DNA, anti nucleosom
es autoantibodies and anti membrane DNA for the diagnosis of SLE. Cell memb
rane associated DNA (mDNA) has been described on B lymphocytes and monocyte
s, but not on T cells. Antibodies to mDNA were identified by an indirect im
munofluorescence assay using a B cell line fixed but notpermeabilised. At a
1:40 serum dilution, anti mDNA is almost associated with the diagnosis of
systemic lupus erythematosus (SLE). Anti mDNA were shown to be different in
specificity as compared with anti double stranded nuclear DNA. We compare
its characteristics as diagnostic procedure to the conventional anti dsDNA
antibody, detection and to the recently introduced anti nucleosome antibody
test documented as associated with SLE. It appears that the best sensitivi
ty (0.65) and specificity (0.98) is given by the antimDNA test.