RFLP analysis for cultivar identification of persimmons

Using cDNA clones for 1-aminocyclopropane-1-carboxylate (ACC) oxidase (ACCO -1) and polygalacturonase. (PG-9) as probes, restriction fragment length po lymorphism (RFLP) was analyzed in order to fingerprint and identify Japanes e persimmon cultivars. In general, a high degree of polymorphism was reveal ed among Japanese persimmon cultivars. However, only a low degree of polymo rphism was observed between the fast-maturing type-B line and the late-matu ring Izumo, Iwami, and M-2 lines derived from the 'Saijo' cultivar. We have demonstrated that RFLP analysis using the probes of ACCO-1 and PG-9 clones is an efficient and convenient method for the classification and discrimin ation of 'Saijo' lines and of main Japanese persimmon cultivars. (C) 2001 E lsevier Science B.V. All rights reserved.