Solid phase synthesis of DNA-3 '-PNA chimeras by using Bhoc/Fmoc PNA monomers

Oligonucleotides carrying a peptide nucleic acid (PNA) tail at the 3'-end h ave been efficiently prepared by an on-line automated synthetic protocol ex ploiting commercially available Bhoc/Fmoc PNA monomers for the assembly of the PNA tract, followed by a deprotection/reprotection of the base protecti ng groups. The syntheses of the ODN domain in the chimeras have then been p erformed by standard methods. The hybridization properties of the synthesiz ed chimeras with complementary DNA fragments have been investigated by ther mal denaturation experiments. (C) 2001 Elsevier Science Ltd. All rights res erved.