Phytochemical profiles of Targionia lorbeeriana: Grown in vitro and in theopen

The terpenoid constituents of Targionia lorbeeriana grown in vivo and in vi tro were compared. The analysis of the dichloromethane extract was performe d by HPLC-UV and by HPLC-MS. The obtained results revealed that the sesquit erpene lactones isolated from the dichloromethane extract of the wild Targi onia lorbeeriana were also produced by the liverwort in in vitro cultures, in the same relative amounts. The composition of essential oils was evaluated by GC and GC-MS. Both, the yield and diversity of the essential oil obtained from wild growing T. lorb eeriana gametophytes were higher than those growing in vitro. Although, a s ignificant number of compounds produced in vivo were maintained in vitro, a considerable number of other ones were not detected. Instead, under in vit ro conditions, some new compounds were found which do not accumulate under wild conditions.