A comparison of three site-directed mutagenesis kits

In this comparative study, three different mutagenesis kits, namely the Mut aGene phagemid in vitro mutagenesis kit (Bio-Rad), the Transformera Site-Di rected mutagenesis kit (Clontech) and the Quik-change site-directed mutagen esis kit (Stratagene) were used for the mutagenesis of IPNS genes. However, a large difference in mutation efficiencies among these kits was encounter ed. Furthermore, these kits employ different strategies with its own indivi dual strengths and weaknesses. Thus, a comparison among these three kits to evaluate their usefulness and improvements on the strategy adopted by the Quik-change site-directed mutagenesis kit, which was the kit of choice for our work, are presented for the benefit of research work.