Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis

Authors
Fukusaki, E Oishi, T Tanaka, H Kajiyama, S Kobayashi, A
Citation
E. Fukusaki et al., Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis, Z NATURFO C, 56(9-10), 2001, pp. 814-819
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
ISSN journal
09395075 → ACNP
Volume
56
Issue
9-10
Year of publication
2001
Pages
814 - 819
Database
ISI
SICI code
0939-5075(200109/10)56:9-10<814:IOGIBT>2.0.ZU;2-F
Abstract
The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by t axol treatment of HeLa cells. Forty-eight cDNA clones were differentially d isplayed through the experiments. The cDNA fragments obtained were separate ly spotted onto glass slides to prepare a tailor-made DNA chip. The gene ex pression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.