Interaction between the plasma membrane and aggregate lipid surface determi
nes how efficiently the encapsulated drug will be delivered into the cell.
Electrostatic interactions are one of the main forces affecting liposome an
d aggregate association with the charged cell surface. In this study, the e
ffect of surface charge on the association of liposomes with human colon CX
-1.1 cancer cells was studied. When phosphatidylserine was incorporated int
o a lipid bilayer, the amount of liposomes associated with cells tended to
increase along with the amount of negatively charged lipid present in the l
iposomal lipid bilayer. When the cationic lipid dioleoyl-1,2-diacyl-3-trime
thylammonium-propane (DOTAP) was included into the liposome formula, their
uptake by the cells was also increased. Maximum binding occurred when the a
mount of positively charged lipids in liposomes was about 10 mol% of lipids
.