Effect of the composition of culture media on the laccase production of Lentinus edodes strains

Citation
H. Morais et al., Effect of the composition of culture media on the laccase production of Lentinus edodes strains, ACT BIOTECH, 21(4), 2001, pp. 307-320
Citations number
31
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
ACTA BIOTECHNOLOGICA
ISSN journal
01384988 → ACNP
Volume
21
Issue
4
Year of publication
2001
Pages
307 - 320
Database
ISI
SICI code
0138-4988(2001)21:4<307:EOTCOC>2.0.ZU;2-4
Abstract
The effect of charge, ion radii and concentration of cations and fermentati on time on the laccase production of four Lentinus edodes strains was deter mined using 28 different fermentation media and 60 days of fermentation tim e. Samples were taken every 10 days and the laccase activity was determined by visible spectrophotometry. Principal component analysis (PCA) was used for the assessment of similarities and dissimilarities between the laccase activities of the samples. As PCA is not suitable for the separation of the strength (potency) and selectivity of the effect of various factors (compo sition of cultures, fermentation time, Lentinus edodes strains) on the lacc ase production, they were separated by the spectral mapping technique (SPM) . The dimensionality of the matrices of PC loadings and variables and the s electivity maps were reduced to two by the lion-linear mapping technique. T he results of PCA and SPM were compared by calculating linear relationships among the potency values and the corresponding coordinates of PCA and SPM maps. It was established that neither the type of the cations nor their con centration in the fermentation media had a significant effect on the laccas e production. It was found that the type of the Lentinus edodes strains and the fermentation time exerts a considerable effect both on the strength an d on the selectivity of the laccase production. It was further proven that the results of PCA and SPM were considerably different. Therefore, their si multaneous application in future quantitative structure activity relationsh ip (QSAR) studies is highly recommended.