Expression of CD3 and CD11b antigens on blood and mammary gland leukocytesand bacterial survival in milk of cows with experimentally induced Staphylococcus aureus mastitis

Objectives-To differentiate early (1 to 8 days) from late (9 to 14 days) in flammatory phases and assess relationships between leukocyte phenotype and bacterial recovery in cows with Staphylococcus aureus-induced mastitis. Animals-10 first-lactation Holstein cows. Procedure-Blood and milk samples were collected from 4 or 6 cows before and after intramammary infusion of sterile broth or S aureus, respectively. Fl ow cytometric expression of CD3 and CD11b antigens on blood and milk leukoc ytes, leukocyte differential counts, bacterial counts in milk, and somatic cell counts were determined longitudinally. Results-Density of CD3 molecules decreased on blood lymphocytes and increas ed on milk lymphocytes after infusion of bacteria. Density of CD11b molecul es on lymphocytes and phagocytes and percentage of CD11b(+) lymphocytes in milk increased significantly after infusion; maximum values were achieved d uring the early inflammatory phase. Density of CD3 and CD11b molecules on m ilk lymphocytes and macrophages, respectively, 1 day after inoculation were negatively correlated with bacterial recovery on day 1 and days 9 to 14, r espectively. Density of CD11b molecules on milk macrophages and the ratios of phagocyte to lymphocyte percentages and polymorphonuclear cell to macrop hage percentages in milk differentiated the early from the late inflammator y phase. Conclusions and Clinical Relevance-Activation of bovine mammary gland macro phages and T cells in response to intramammary infusion of S aureus was ass ociated with an inability to culture this bacterium from milk. Identificati on of specific inflammatory phases of S aureus-induced mastitis in cows may allow for the design of more efficacious treatment and control programs.