Partial cDNA sequence analysis of the boar proacrosin-binding protein

A partial cDNA sequence of the boar 28, 29-kDa proacrosin-binding protein w as determined. The desired sequence was amplified and obtained by RT-PCR fo llowed by nested PCR. The oligonucleotide primers for RT-PCR were designed by deduction from the internal amino acid sequence of the boar proacrosin-b inding protein. An approximately 1-kbp DNA fragment was analysed by agarose gel electrophoresis, in which the boar testicular total RNA or poly(A)(+) RNA was used as a template for RT-PCR followed by nested PCR. This PCR prod uct was inserted into a TA vector. The DNA sequence of the inserted PCR pro duct was then determined by the chain termination method.