Effect of atorvastatin on intracellular calcium uptake in coronary smooth muscle cells from diabetic pigs fed an atherogenic diet

Intracellular Ca2+ store loading has been shown to alter proliferation and apoptosis of several cell types. In addition, HMG-CoA reductase inhibitors (i.e. atorvastatin) are effective in treating diabetic dyslipidemic patient s. Thus, we hypothesized that chronic atorvastatin treatment would prevent increased Ca2+ uptake into intracellular Ca2+ stores in vascular smooth mus cle cells from diabetic dyslipidemic pigs. Male Yucatan pigs were divided i nto four groups for 20 weeks - (1) low fat fed (control); (2) hyperlipidemi c (F), (3) alloxan-induced diabetic dyslipidemic (DF); and (4) diabetic dys lipidemic pigs treated with atorvastatin (DFA). The F, DF, and DFA groups w ere fed a high fat/cholesterol diet. Cells were isolated from the coronary artery and the rnyoplasmic Ca2+ (Ca-m) response measured using single cell fura-2 imaging. The Ca-m response to caffeine (5 mM to release Ca2+ from th e sarcoplasmic reticulum, SR) and ionomycin (10 muM; to release the total C a2+ store) was determined in either the presence of low Na (19Na; inhibits Na+-Ca2+ exchange), thapsigargin (TSG; inhibits the SR Ca2+ pump), and a 19 Na + TSG solution. Low Na induced the uptake of Ca2+ into both SR and non-S R Ca2+ stores in the DF group, but not the DFA group. Furthermore, after de pletion of the SR Ca2+ store with TSG, 19Na evoked Ca2+ uptake into non-SR Cal + stores in all three groups except in the DFA group. In summary, this study demonstrates that atorvastatin prevents the enhanced uptake of Ca2+ b y SR and non-SR Ca2+ stores in diabetic dyslipidemic pigs. (C) 2001 Elsevie r Science Ireland Ltd. All rights reserved.