F. Brambillasca et al., Promoter analysis of TFPT (FB1), a molecular partner of TCF3 (E2A) in childhood acute lymphoblastic leukemia, BIOC BIOP R, 288(5), 2001, pp. 1250-1257
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We previously identified the TFPT (FB1) gene as a molecular partner of TCF3
(E2A) in childhood pre-B cell acute lymphoblastic leukemia (ALL). TFPT (FB
1) alignment in man, mouse and rat displays a very high degree of identity,
indicating that it may play a basic role in mammalian cells. To get insigh
ts into this role, we have identified and studied the TFPT (FB1) promoter a
nd its responsiveness to hematopoietic transcriptional factors. We found th
at the TFPT (FB1) 5' flanking sequence displays the features of a TATA-less
promoter with weak homology to Inr (Initiator) elements. Starvation experi
ments suggested that TFPT (FB1) expression might be constitutive. Neverthel
ess, the TFPT (FB1) promoter, tested by transactivation assays, was found t
o be responsive to Ikaros 2 and, mainly, to PU.1, a transcription factor be
longing to the Ets family. Thus, these hematopoietic factors, known to play
critical roles during the early stages of B cell differentiation and to be
involved in leukemia, might modulate TFPT (FB1) expression during hematopo
iesis and/or leukemia development. (C) 2001 Academic Press.