Tyrosine phosphorylation of beta-dystroglycan at its WW domain binding motif, PPxY, recruits SH2 domain containing proteins

Citation
F. Sotgia et al., Tyrosine phosphorylation of beta-dystroglycan at its WW domain binding motif, PPxY, recruits SH2 domain containing proteins, BIOCHEM, 40(48), 2001, pp. 14585-14592
Citations number
38
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMISTRY
ISSN journal
00062960 → ACNP
Volume
40
Issue
48
Year of publication
2001
Pages
14585 - 14592
Database
ISI
SICI code
0006-2960(200112)40:48<14585:TPOBAI>2.0.ZU;2-C
Abstract
beta -Dystroglycan is a ubiquitously expressed integral membrane protein th at undergoes tyrosine phosphorylation in an adhesion-dependent manner. Howe ver, it remains unknown whether tyrosine-phosphorylated beta -dystroglycan interacts with SH2 domain containing proteins. Here, we show that the tyros ine phosphorylation of l3-dystroglycan is constitutively elevated in v-Src transformed cells. We next reconstituted this phosphorylation event in vivo by transiently coexpressing wild-type c-Src with a fusion protein containi ng full-length beta -dystroglycan. Our results demonstrate that Src-induced tyrosine phosphorylation of beta -dystroglycan is strictly dependent on th e presence of a PPxY motif at its extreme C-terminus. In the nonphosphoryla ted state, this PPxY motif is normally recognized as a ligand by the WW dom ain; phosphorylation at this site blocks the binding of certain WW domain c ontaining proteins. Using a GST fusion protein carrying the cytoplasmic tai l of beta -dystroglycan, we identified five SH2 domain containing proteins that interact with beta -dystroglycan in a phosphorylation-dependent manner , including c-Src, Fyn, Csk, NCK, and SHC. We localized this binding activi ty to the PPxY motif by employing a panel of beta -dystroglycan-derived pho sphopeptides. In addition, tyrosine phosphorylation of beta -dystroglycan i n vivo resulted in the coimmunoprecipitation of the same SH2 domain contain ing proteins, and this binding event required the -dystroglycan C-terminal PPxY motif. We discuss the possibility that tyrosine phosphorylation of the PPxY motif within beta -dystroglycan may act as a regulatory switch to inh ibit the binding of certain WW domain containing proteins, while recruiting SH2 domain containing proteins.