Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter

Authors
Bai, LQ Collins, JF Xu, H Xu, LP Ghishan, FK
Citation
Lq. Bai et al., Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter, BBA-GENE ST, 1522(1), 2001, pp. 42-45
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1522
Issue
1
Year of publication
2001
Pages
42 - 45
Database
ISI
SICI code
0167-4781(20011111)1522:1<42:MCOAMT>2.0.ZU;2-K
Abstract
We report the novel cloning and preliminary characterization of a murine ty pe III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/ +61, -6001+61 and -225/+61, showed significant luciferase activity (6-14-fo ld over background) when transfected into human colon carcinoma (Caco-2) an d opossum kidney (OKP) cells. (C) 2001 Elsevier Science B.V. All rights res erved.