ELF magnetic field inhibits gap junctional intercellular communication andinduces hyperphosphorylation of connexin43 in NIH3T3 cells

The effects of extremely low frequency (ELF) magnetic field on gap junction al intercellular communication (GJIC), protein levels, and phosphorylation of connexin43 (Cx43) were studied in NIH3T3 cells. The suppression of GJIC by 24 h, 50 Hz, 0.8 mT ELF magnetic field, 2 h, 3 ng/ml 12-O-tetradecanoylp horbol-13-acetate (TPA), or ELF combined with TPA treatment was confirmed b y the fluorescence recovery after photobleaching (FRAP) analysis with a con focal microscope. The results showed that ELF or TPA exposure induced 50-60 % inhibition of GJIC (P < 0.01). ELF combined with TPA enhanced the inhibit ion of GJIC. Western blot analysis using Cx43 specific antibodies showed ob viously decreasing non phosphorylated Cx43 (P-0) induced by ELF and/or TPA exposure. On the other hand, cells treated with ELF and/or TPA displayed a hyperphosphorylated Cx43 band (P-3). However, there was no obvious changes in the level of Cx43 protein. The results implied that the P-3 band appeare d to result from phosphorylation of P-0. But it remains possible that upon the ELF exposure P-0 is converted to P-1, P-2 or both and that P-3 is forme d from P-1 or P-2 resulting in the observed hyperphosphorylation pattern. F rom the present study, we conclude that ELF magnetic field inhibits GJIC an d the main mechanism is the hyperphosphorylation of Cx43. (C) 2001 Wiley-Li ss, Inc.