Effect of four acrylic bone cements on transforming growth factor-beta 1 expression by osteoblast-like cells MG63

Based on the hypothesis that bone cements cause changes in the production o f transforming growth factor-beta I (TGF-beta1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were exami ned using the osteoblast-like cell line MG63. The extracts in MEM of the ce ments were tested, following 1 h- and 7 day-curing. MG63 cells seldom expre ssed mRNA specific for TGF-beta1 in basal conditions. The cultures expresse d mRNA constantly after incubation with the extract of CMW I cured for I h. TGF-beta1 specific mRNA was seldom expressed after incubation with the oth er cement extracts. The release of TGF-beta1 into the conditioned medium wa s increased significantly by CMW I extract at I h-curing, but was not chang ed significantly by CMW I extract at 7 day-curing and by the extracts of th e other cements, at both curing times, The stimulating effect of CMW I on t he secretion of TGF-beta1, even with all the restrictions of an in vitro st udy of continuous cell lines, if confirmed in vivo, might favor the develop ment of the synovial-like membrane around the implant, and therefore impair the chance of success of the prosthesis. (C) 2001 Elsevier Science Ltd. Al l rights reserved.