Fluorescent monitoring of kinase activity in real time: Development of a robust fluorescence-based assay for abi tyrosine kinase activity

Fluorescent biosensors hold great promise for drug discovery. Using a solid -phase version of protein semi-synthesis. we incorporated two fluorophores at specific sites within a truncated version of the c-Crk-II protein. The r esulting fluorescent protein biosensor permits the real-time monitoring of Abl kinase activity and provides a robust and rapid method for assaying Abl kinase inhibitors. (C) 2001 Elsevier Science Ltd. All rights reserved.