Hodgkin-Reed Sternberg (H-RS) cells harbour clonal immunoglobulin gene (Ig)
rearrangements in almost all cases of classical Hodgkin's disease but lack
Ig gene expression. In the H-RS cell line L1236. a somatic mutation of the
Ig heavy-chain gene promoter octamer motif has been described as a putativ
e reason for absence of Ig gene expression. We addressed transcriptional ac
tivity of this mutated promoter by performing reporter gene studies and gel
retardation assays. The results showed that the mutation outside the codin
g region of the rearranged Ig gene in L1236 cells leads to downregulation o
f Ig gene expression in H-RS cells.