Deregulation of immunoglobulin gene transcription in the Hodgkin-Reed Sternberg cell line L1236

Citation
D. Re et al., Deregulation of immunoglobulin gene transcription in the Hodgkin-Reed Sternberg cell line L1236, BR J HAEM, 115(2), 2001, pp. 326-328
Citations number
13
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
00071048 → ACNP
Volume
115
Issue
2
Year of publication
2001
Pages
326 - 328
Database
ISI
SICI code
0007-1048(200111)115:2<326:DOIGTI>2.0.ZU;2-J
Abstract
Hodgkin-Reed Sternberg (H-RS) cells harbour clonal immunoglobulin gene (Ig) rearrangements in almost all cases of classical Hodgkin's disease but lack Ig gene expression. In the H-RS cell line L1236. a somatic mutation of the Ig heavy-chain gene promoter octamer motif has been described as a putativ e reason for absence of Ig gene expression. We addressed transcriptional ac tivity of this mutated promoter by performing reporter gene studies and gel retardation assays. The results showed that the mutation outside the codin g region of the rearranged Ig gene in L1236 cells leads to downregulation o f Ig gene expression in H-RS cells.