Ca. Bunnell et al., Phase I clinical trial of 7-cyanoquinocarcinol (DX-52-1) in adult patientswith refractory solid malignancies, CANC CHEMOT, 48(5), 2001, pp. 347-355
Purpose: A phase I study of the antitumor antibiotic 7-cyanoquinocarcinol,
DX-52-1, was conducted in patients with refractory solid malignancies. This
study sought to determine the maximum tolerated dose and principal toxicit
ies of this agent and to characterize its pharmacokinetic behavior. Methods
: Patients were required to have adequate bone marrow, renal and hepatic fu
nction. DX-52-1 was administered by i.v. continuous infusion over a 6-h per
iod each week for four consecutive weeks followed by a 2-week rest period,
which constituted one cycle of treatment. Results: Initial dose levels were
3, 6, and 10 mg/m(2). An intermediate dose level of 8 mg/m(2) was added af
ter acceptable toxicity was observed at the 6 mg/m dose level, but doselimi
ting toxicities, including life-threatening ones, were seen at the 10 mg/m(
2) dose level in all three patients. The maximum tolerated dose (MTD) was s
ubsequently determined to be 6 mg/m(2). Because a clear pattern of toxiciti
es was not initially evident, a larger than usual number of additional pati
ents (16) were enrolled at the MTD to better distinguish toxicities due to
the study drug from those secondary to the patients' underlying malignancie
s. Even at the MTD, the drug was poorly tolerated, with gastrointestinal to
xicities (abdominal pain, nausea, vomiting and increased liver function tes
ts) predominating and dose-limiting. Pharmacokinetic studies revealed that
the mean maximum plasma concentration of DX-52-1 in patients evaluated at t
he MTD (138.8 +/- 59.3 ng/ml, n = 19) was considerably lower than the conce
ntrations required for cytostatic or cytotoxic activity against sensitive h
uman tumor cell lines in vitro. Further, the weekly dose intensity of the m
ost efficacious treatment schedule identified during in vivo antitumor effi
cacy studies was 60 times greater than the 6 mg/m(2) weekly dose tolerated
by cancer patients. None of the 33 patients participating in this study, in
cluding the 22 patients evaluated at the MTD, had any response to treatment
. Conclusion: Given the poor tolerability, the inability to achieve drug le
vels necessary to inhibit in vitro or in vivo tumor growth, and the lack of
any responses in our study, DX-52-1, as given by this schedule, does not a
ppear to warrant further investigation in phase II studies.