Large-scale solution synthesis of phosphorothioate oligonucleotides: A comparison of the phosphoroamidite and phosphotriester dimeric approaches

Two different approaches to the solution synthesis of oligonucleotides are compared. Both procedures use dimer synthons as building blocks to save tim e and to improve the final product yield and quality. These strategies look quite promising due to their easy upscaling, providing an advantageous alt ernative to the well-established solid-phase methods.