Compaction, stainability and methylation of the late replicating X chromosome in mouse female fibroblasts

Authors
Bernardino-Sgherri, J Dutrillaux, B
Citation
J. Bernardino-sgherri et B. Dutrillaux, Compaction, stainability and methylation of the late replicating X chromosome in mouse female fibroblasts, CYTOG C GEN, 94(1-2), 2001, pp. 79-81
Citations number
7
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CYTOGENETICS AND CELL GENETICS
ISSN journal
03010171 → ACNP
Volume
94
Issue
1-2
Year of publication
2001
Pages
79 - 81
Database
ISI
SICI code
0301-0171(2001)94:1-2<79:CSAMOT>2.0.ZU;2-U
Abstract
In metaphases from female mouse fibroblasts, successive stainings by Giemsa and DAPI and immunolabeling of 5-methylcytosine were performed with or wit hout bromodeoxyuridine pretreatment. It was shown that, compared to all oth er chromosomes, the late replicating X is the least methylated, the most co mpacted, and the most intensely stained by DAPI and Giemsa. Copyright (C) 2 001 S. Karger AG, Basel.