Waardenburg-Shah syndrome combines the reduced enteric nervous system chara
cteristic of Hirschsprung's disease with reduced pigment cell number, altho
ugh the cell biological basis of the disease is unclear. We have analysed a
zebrafish Waardenburg-Shah syndrome model. We show that the colourless gen
e encodes a sox10 homologue, identify sox10 lesions in mutant alleles and r
escue the mutant phenotype by ectopic sox10 expression. Using iontophoretic
labelling of neural crest cells, we demonstrate that colourless mutant neu
ral crest cells form ectomesenchymal fates. By contrast, neural crest cells
which in wild types form non-ectomesenchymal fates generally fail to migra
te and do not overtly differentiate. These cells die by apoptosis between 3
5 and 45 hours post fertilisation. We provide evidence that melanophore def
ects in colourless mutants can be largely explained by disruption of nacre/
mitf expression. We propose that all defects of affected crest derivatives
are consistent with a primary role for colourless/sox10 in specification of
non-ectomesenchymal crest derivatives. This suggests a novel mechanism for
the aetiology of Waardenburg-Shah syndrome in which affected neural crest
derivatives fail to be generated from the neural crest.