Zebrafish colourless encodes sox10 and specifies non-ectomesenchymal neural crest fates

Citation
Ka. Dutton et al., Zebrafish colourless encodes sox10 and specifies non-ectomesenchymal neural crest fates, DEVELOPMENT, 128(21), 2001, pp. 4113-4125
Citations number
64
Categorie Soggetti
Cell & Developmental Biology
Journal title
DEVELOPMENT
ISSN journal
09501991 → ACNP
Volume
128
Issue
21
Year of publication
2001
Pages
4113 - 4125
Database
ISI
SICI code
0950-1991(200111)128:21<4113:ZCESAS>2.0.ZU;2-L
Abstract
Waardenburg-Shah syndrome combines the reduced enteric nervous system chara cteristic of Hirschsprung's disease with reduced pigment cell number, altho ugh the cell biological basis of the disease is unclear. We have analysed a zebrafish Waardenburg-Shah syndrome model. We show that the colourless gen e encodes a sox10 homologue, identify sox10 lesions in mutant alleles and r escue the mutant phenotype by ectopic sox10 expression. Using iontophoretic labelling of neural crest cells, we demonstrate that colourless mutant neu ral crest cells form ectomesenchymal fates. By contrast, neural crest cells which in wild types form non-ectomesenchymal fates generally fail to migra te and do not overtly differentiate. These cells die by apoptosis between 3 5 and 45 hours post fertilisation. We provide evidence that melanophore def ects in colourless mutants can be largely explained by disruption of nacre/ mitf expression. We propose that all defects of affected crest derivatives are consistent with a primary role for colourless/sox10 in specification of non-ectomesenchymal crest derivatives. This suggests a novel mechanism for the aetiology of Waardenburg-Shah syndrome in which affected neural crest derivatives fail to be generated from the neural crest.