Ikaros expression as a marker for lymphoid progenitors during zebrafish development

Citation
Ce. Willett et al., Ikaros expression as a marker for lymphoid progenitors during zebrafish development, DEV DYNAM, 222(4), 2001, pp. 694-698
Citations number
32
Categorie Soggetti
Cell & Developmental Biology
Journal title
DEVELOPMENTAL DYNAMICS
ISSN journal
10588388 → ACNP
Volume
222
Issue
4
Year of publication
2001
Pages
694 - 698
Database
ISI
SICI code
1058-8388(200112)222:4<694:IEAAMF>2.0.ZU;2-L
Abstract
The Ikaros gene encodes a transcription factor that, in mice, has been show n to be essential for the correct differentiation of B and T lymphocytes an d is expressed in all cells of the lymphoid lineage, including pluripotent hematopoietic stem cells. During development in zebrafish, Ikaros expressio n begins in lateral mesoderm, and continues in the intermediate cell mass ( ICM), which is derived from lateral mesoderm and has been shown to generate primitive hematopoietic precursors. Cells expressing Ikaros were then seen on the ventral side of the dorsal aorta, known to be a location of definit ive hematopoietic precursors in birds and mammals. Ikaros-expressing cells were also found in the pharyngeal region, near the forming thymus. Later, s uch cells were seen in the pronephros, the site of hematopoiesis in adult f ish. The timing of appearance of Ikaros-expressing cells suggests that, sim ilar to other vertebrates, lymphocytes in the thymus arise from hematopoiet ic tissue located near the dorsal aorta or in the ICM. (C) 2001 Wiley-Liss, Inc.