A hybrid bacterial replication origin

Authors
Seitz, H Welzeck, M Messer, W
Citation
H. Seitz et al., A hybrid bacterial replication origin, EMBO REP, 2(11), 2001, pp. 1003-1006
Citations number
22
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO REPORTS
ISSN journal
1469221X → ACNP
Volume
2
Issue
11
Year of publication
2001
Pages
1003 - 1006
Database
ISI
SICI code
1469-221X(200111)2:11<1003:AHBRO>2.0.ZU;2-M
Abstract
We constructed a hybrid replication origin that consists of the main part o f oriC from Escherichia coli, the DnaA box region and the AT-rich region fr om Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli D naA protein, and the DnaB helicase was loaded into the single-stranded bubb le. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.