The application of sewage sludge to agricultural land brings several chemic
als to the soil ecosystem. Linear alkylbenzene sulfonates (LAS) and nonylph
enol (NP) are frequently found in sludge at relatively high concentrations.
The toxic effects of LAS and NP to two soil invertebrates (Folsomia candid
a and Enchytraeus albidus) and five different microbial processes (aerobic
respiration, nitrification, denitrification, anaerobic CH, production, and
anaerobic CO2 production) were investigated in sludge-soil mixtures. Median
lethal concentrations (LC50 values) and median effective concentrations (E
C50 values) were quite similar and calculated to be in the range of 1,143 t
o 1,437 and 71 to 437 mg/kg (dry wt) for LAS and NP, respectively. The EC50
values for nitrification and CH, production were 431 and 277 mg/kg, respec
tively, for LAS and 343 and 754 mg/kg, respectively, for NP Aerobic respira
tion and denitrification were not inhibited at the tested concentrations. T
he results show that NP was more toxic than LAS to both F. candida and E. a
lbidus, whereas LAS was more toxic than NP to the anaerobic CH, production.
The safety margins between the lowest 10% effective concentration (EC10) a
nd the estimated environmental concentration were a factor of l 1 for LAS a
nd 510 for NP when the concentrations of the contaminants corresponded to t
he current Danish cutoff values of 1,300 mg/kg for LAS and 30 mg/kg for NP.