PYRIDINE NUCLEOTIDE-DEPENDENT CHANGES IN THE PHASE-PARTITIONING BEHAVIOR OF NADP-LINKED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PEA-CHLOROPLASTS

Authors
ANDERSON LE LI D PACOLD MM
Citation
Le. Anderson et al., PYRIDINE NUCLEOTIDE-DEPENDENT CHANGES IN THE PHASE-PARTITIONING BEHAVIOR OF NADP-LINKED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PEA-CHLOROPLASTS, PLANT SCI, 99(1), 1994, pp. 39-42
Citations number
8
Categorie Soggetti
Plant Sciences
Journal title
PLANT SCIENCE
ISSN journal
01689452 → ACNP
Volume
99
Issue
1
Year of publication
1994
Pages
39 - 42
Database
ISI
SICI code
0168-9452(1994)99:1<39:PNCITP>2.0.ZU;2-A
Abstract
Addition of NAD(H) or NADP(H) to an aqueous two-phase polyethylene gly col-dextran system affects the partitioning of purified pea (Pisum sat ivum L.) chloroplast glyceraldehyde-3-P dehydrogenase. The results ind icate that the nature of the bound pyridine nucleotide affects the con formation and the state of aggregation of the enzyme, as suggested ear lier by size exclusion (R. Cerff, fur. J. Biochem., 82 (1978) 45-53) a nd sucrose density gradient centrifugation (P. Pupillo and G. Giuliani Picccari, fur. J. Biochem., 51 (1975) 475-482) experiments.