Spinal cord metabolism during thoracic aortic cross-clamping in pigs with special reference to the effect of allopurinol

Objective: investigate the metabolic response of the spinal cord and the ef fect of allopurinol following cross clamping of the descending thoracic aor ta in a porcine model. Design: experimental animal study. Materials: twelve domestic swine. Six pigs were pre-treated with allopurino l, while six pigs served as controls. Methods: measurement of extracellular concentrations of glucose, pyruvate, lactate, glycerol and glutamate using microdialysis in the lumbar spinal co rd. Measurement of lumbar spinal blood flow using laser Doppler technique. Results: for all animals there was a significant decrease in concentrations of glucose and pyruvate together with a significant increase in the lactat e-pyruvate ratio during aortic cross clamping. There was also a significant increase in glycerol concentrations 60 min after cross clamping, and a sig nificant decrease in glutamate concentrations after 50 min. No differences in concentrations of glucose, pyruvate, lactate and glutamate or the glutam ate-pyruvate ratio were observed between animals used as controls and those treated with allopurinol. The laser Doppler flux decreased to 40% of pre c ross-clamp level, returning to normal values at declamping. Conclusion: the changes in energy-related metabolites reflect a considerabl e ischaemia in the spinal cord tissue but there was no convincing effect of allopurinol on the lumbar spinal cord metabolism during thoracic aortic cr oss clamping in this model.