Conformational and functional significance of residue proline 17 in chicken muscle adenylate kinase

The effect of mutation proline 17 on the multiple conformations and catalyt ic function in chicken muscle aden) late kinase (AK) has been studied. The substitution of proline 17 with glycine or valine altered the distribution of multiple conformations. Compared with the wild-type enzyme, the P17G and P17V mutants contained decreased fraction of minor conformer from 18% to 9 % and 11%, respectively. Due to the mutation, the enzyme showed lower secon dary structural content, poorer affinity to substrates or substrate analogu es, and reduced catalytic efficiency. The results revealed the significance of proline 17 in the conformation and function of AK. (C) 2001 Published b y Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.