NF-kappa B is required for TNF-alpha-directed smooth muscle cell migration

Citation
Zb. Wang et al., NF-kappa B is required for TNF-alpha-directed smooth muscle cell migration, FEBS LETTER, 508(3), 2001, pp. 360-364
Citations number
39
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
508
Issue
3
Year of publication
2001
Pages
360 - 364
Database
ISI
SICI code
0014-5793(20011123)508:3<360:NBIRFT>2.0.ZU;2-Z
Abstract
Migration of vascular smooth muscle cells (VSMC) is a crucial event in the formation of vascular stenotic lesions. Tumor necrosis factor-alpha (TNF-al pha) is elaborated by VSMC in atherosclerosis and following angioplasty. We investigated the role of nuclear factor-kappaB (NF-kappaB) in human VSMC m igration induced by TNF-alpha. Adenoviral expression of a mutant form of th e inhibitor of NF-kappaB, I kappaB-alphaM, suppressed TNF-alpha -triggered degradation of cellular I kappaB-alpha, inhibited activation of NF-kappaB, and attenuated TNF-alpha -induced migration. Further, I kappaB-alphaM suppr essed TNF-alpha -stimulated release of interleukin-6 and -8 (IL-6 and IL-8) . Neutralization of IL-6 and IL-8 with appropriate antibodies reduced TNF-a lpha -induced VSMC migration. Addition of recombinant IL-6 and IL-8 stimula ted migration. Collectively, our data provide initial evidence that TNF-alp ha -mediated VSMC migration requires NF-kappaB activation and is associated with induction of IL-6 and IL-8 which act in an autocrine manner. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Scienc e B.V. All rights reserved.