A novel approach to search for identity by descent in small samples of patients and controls from the same Mendelian breeding unit: A pilot study on myopia
S. Heath et al., A novel approach to search for identity by descent in small samples of patients and controls from the same Mendelian breeding unit: A pilot study on myopia, HUMAN HERED, 52(4), 2001, pp. 183-190
Autosomal dominant high myopia, a genetic disorder already mapped to region
18p 11.31, is common in Carloforte (Sardinia, Italy), an isolated village
of 8,000 inhabitants descending from a founder group of 300 in the early 17
00s. Fifteen myopic propositi and 36 normal controls were selected for not
having ancestors in common at least up to the grandparental generation, alt
hough still descendants of the original founders. All subjects were genotyp
ed for 14 markers located on autosome 18 at a resolution of about 10 cM. Al
lelic distributions were found to be similar at all tested loci in proposit
i and controls, except for the candidate marker D18S63 known to segregate i
n close linkage association with high myopia. In particular, the frequency
of allele 85 among the propositi was almost double that of the controls (Fi
sher's exact test, p = 0.037). The association is more striking when the fr
equency of the genotype 85/85 in the two groups is compared Fisher's exact
test, p = 0.005). This conclusion was further evaluated through a bootstrap
analysis by computing the overall probability of the observed data under t
he null hypothesis (i.e. no difference between the two groups in frequency
distributions for the chromosome 18 markers). Again, marker D18S63 was foun
d to have a sample probability lower than 0.004, which is significant at th
e 0.05 level after correcting for simultaneous testing of multiple loci. Th
e study demonstrates the efficiency of our novel strategy to detect identit
y by descent (IBD) in small numbers of patients and controls when they are
both part of well-defined Mendelian breeding units (MBUs). The iterative ap
plication of our strategy in separate MBUs is expected to become the method
of choice to evaluate the ever-growing number of reported associations bet
ween candidate genes and multifactorial traits and diseases. Copyright (C)
2002 S. Karger AG, Basel.